Axioplan 2 (Zeiss)
Obsah (pouze anglicky):
1) Application of the Zeiss Axioplan 2
2) Instrument specification
3) Booklets, user manuals, and theory of fluorescence microscopy
1) Application of the Zeiss Axioplan 2
2) Instrument specification
3) Booklets, user manuals, and theory of fluorescence microscopy
1) Application of Zeiss Axioplan 2
- Bright-field imaging including DIC
- Dark-field imaging
- Fluorescence imaging
2) Instrument specification
| * available upon request, please contact Hana Sehadova | ||||||
| Description and magnification | N.A. | W.D. (mm) | Cover glass thickness | Immersion | Correcting rings | Condenser |
|---|---|---|---|---|---|---|
| Plan-NEOFLUAR 5x * | 0.15 | 18.5 | 0.17 | DIC II | ||
| Plan- NEOFLUAR 10x | 0.30 | 5.2 | 0.17 | DIC II | ||
| Plan- NEOFLUAR 20x | 0.50 | 1.3 | 0.17 | DIC III | ||
| Plan-APOCHROMAT 40x (Korr) | 0.95 | 0.25 | 0.13-0.21 | Yes | DIC III | |
| Plan-APOCHROMAT 63x * | 1.4 | 0.19 | 0.17 | Oil | - | |
| Plan-APOCHROMAT 63x DIC | 1.4 | 0.19 | 0.17 | Oil | DIC III | |
| Plan-APOCHROMAT 100x | 1.4 | 0.17 | 0.17 | Oil | - | |
| Plan-APOCHROMAT 100x DIC | 1.4 | 0.17 | 0.17 | Oil | DIC III | |
| * available upon request, please contact Hana Sehadova To find the possible dyes for each filter set please use the Zeiss webpage |
||||
| Filter sets | Excitation Filter (nm) | Dichoric Mirror (nm) | Emission Filter (nm) | Position |
|---|---|---|---|---|
| AFanlysentechnik F36-670 DAPI |
D 360/50 | 400 DCLP | D 470/40 | 1 |
| Chroma F31-001 FITC |
D 480/30 | Q 505 LP | D 535/40 | 2 |
| Chroma F31-002 TRITC/Cy3 |
D 540/25 | Q 565 LP | D 605/55 | 3 |
| Chroma F41-008 Cy5 |
HQ 620/60 | Q 660 LP | HQ 700/75 | 4 |
| Zeiss FS-05 CFP, Alexa 430, Fura Red Ca2+, Acridine Orange |
BP 395-440 | FT 460 | LP 470 | 6 |
| Zeiss FS-47 CFP, LysoSensor Green, POPO-1 |
BP 436/20 | FT 455 | BP 480/40 | 7 |
| Zeiss FS-46 YFP, Alexa 488, FITC, Fluorescenin, MitoTracker Green |
BP 500/20 | FT 515 | BP 535/30 | 8 |
| Zeiss FS-49 2x BFP, Alexa 350, DAPI, LysoTracker Blue |
G 365 | FT 395 | BP 445/50 | * |
| Zeiss F46-008 Alexa 568, m Cherry,Texas Red, LysoTracker Red, MitoTracker Red |
BP 560/40 | PB 630/75 | * | |
| Zeiss FS-26 Nile Red, mPlum |
BP 575-625 | FT 645 | BP 660-710 | * |
| Others | |
|---|---|
| Light sources | 100W halogen transmitted light source / 150W Hg fluorescence light source |
| Detector | CCD Olympus DP 73 - 17.28 MP - (about) |
| Software | CellSens Standard - Olympus |
3) Booklets, user manuals, and theory of fluorescence microscopy
