Histology laboratory

Histological unit offers scientists collaboration on research projects that require the following techniques:

• histological staining of paraplast sections (Mallory, hematoxylin-eosin, etc.) of animal and plant tissues
• immunohistochemical antigen detection in whole specimens and paraplast tissue sections
• in situ hybridization of nucleic acids in whole preparations and paraplast tissue sections
• preparation and contrasting of semi-thin sections
• preparation of ultrathin sections for transmission electron microscopy
• imaging of biological material in classical light, fluorescence and confocal microscopes
• imaging of biological material with a unique bioluminescence microscope
• image analysis and preparation of high-quality photographic documentation for publication purposes

We also offer technical equipment for independent use of microtomes, cryostat and ultramicrotome, see price list of the Laboratory of Microscopy and Histology. The ultramicrotome can be used independently only by experienced personnel.

Rules for the use of the Histological unit of the LMH:

1. LMH staff provide creative input and technical support for all research steps, including experimental design, sample preparation, imaging experiments, and data analysis on a co-authorship basis.
2. In all your outputs, please mention in the acknowledgment any work conducted on our facility (with or without the participation of our staff) in the following form:
   "The authors used services of the Czech-BioImaging research infrastructure, specifically the Laboratory of Microscopy and Histology, Biology Centre CAS, supported by project LM2023050 funded by the Ministry of Education, Youth and Sports of the Czech Republic, with the instrumental equipment co-financed by the European Union.”
3. All collaborations and independent use of equipment of the LMH must be agreed in advance by the project leader with the Head of the laboratory Dr. Hana Sehadova and are subject to the valid price list of the LMH. The hourly rate for the use of microscopes is significantly reduced in case of collaboration, see the price list of the LMH.
4. The specific date for the individual use of the equipment of the LMH (microtomes, ultramicrotome, cryostat) must be agreed in advance with the technical staff of the LMH, who will coordinate the times of use of the individual devices so that the techniques used do not overlap.
5. The guest worker leaves the workplace in the condition in which he/she took it. He/she shall immediately report any defects found or incurred in the equipment to the laboratory personnel and shall assume responsibility for any damage to the laboratory equipment caused by improper handling or negligence.

Please note - we do not rent diamond knives for making ultra-thin sections.

Equipment

Microtome Leica RM 2165 Ultramicrotome Leica EM UC

Cryostat Leica CM 1850 UV Microtome Leica RR2235 (2 setups available)

Safety rules and instrument usage:

1. No food and drinks are allowed in microscopy and laboratory rooms.
2. The user is responsible to check the equipment for cleanness and obvious damage before the use.
3. The user is obliged to immediately report problems with the equipment or its unusual behavior to the staff of the LMH.
4. The users are not allowed to create, change or delete user accounts on the instruments at the LMH.
5. All experiments must comply with local regulations, particularly concerning cleaning and disinfection after the experiment.
6. No dangerous chemicals, samples or instrumentation can be brought into the LMH without explicit consent of its staff.
7. All experiments with human and animal material must comply with local ethics regulations.
8. Users must take utmost care to avoid improper instrument usage, e.g., immersion oil must be used only with appropriate objective lenses.
9. At the end of the user experiment, the equipment must be returned in exactly the same condition as it was in when the experiment began. All modifications made to the equipment by the user must be removed.